Swine often exhibit high rates (∼18%) of preimplantation conceptus mortality following either artificial insemination or natural mating ( 6). These results validate the utility of SAGE in the pig and establish an initial model linking gene expression profiles at the pathway level with phenotypic progression from ovoid to filamentous stages of conceptus development. Differential expression of these genes in the steroidogenic and oxidative stress response pathways was confirmed by real-time PCR. Comparisons between the SAGE profiles of ovoid and filamentous conceptuses revealed increased expression of key genes in the steroidogenesis and oxidative stress response pathways. Nucleotide sequence alignment searches on public databases provided SAGE tag annotation and gene ontology assignments. Statistical analysis of tag frequencies revealed the differential expression of 431 tags between libraries ( P < 0.05). Serial analysis of gene expression (SAGE) libraries were constructed from in vivo derived ovoid and filamentous swine conceptuses to yield a total of 42,389 tags (ovoid) and 42,391 tags (filamentous) representing 14,464 and 13,098 putative unique transcripts, respectively. The objective of this study was to establish comparative transcriptome profiles of D11 ovoid and D12 filamentous conceptuses and thereby identify temporally regulated genes essential for developmental progression during conceptus elongation. Elongation of the porcine conceptus is a key stage of development during which maternal recognition of pregnancy, initial placental development, and preparation for implantation occurs. Significant conceptus loss occurs during trophectoderm elongation between gestational day 11 ( D11) and day 12 ( D12).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |